MICROBIAL EXAMINATION OF SPOILT AVOCADO FRUIT

ABSTRACT Microbial examination of spoilt Avocado fruits was carried out to obtained the causal organism.  A total of 60 avocado from Ogbete Main market and New market Enugu were examined by culturing on nutrient agar and sabourand dextrose agar (SM), for bacteria and fungi causing post – harvest spoilage on the fruits; six bacterial species and six fungal species were isolated. The bacteria include Erwinia SP  (66.7%), Streptococcus sp,  *25%), E. Coli (18.9%) and  staphycoccus sp (33.3%), Pseudomonas sp (20%) and Chromobacterium sp (5%) has the lowest percentage occurrence, Erwinia sp,  Pseudomonas sp and chromobacterium sp were found to be absent in the control.  The fungi species, includes Aspergillus sp  (50%), Pericillium sp  (25%), Fusarium sp  (16.6%), Rhisopus sp  (13.3%), currularia sp (8.3%) and Rhoctorula sp  (3.3%), were also kolated Avocado fruits from Ogbete main market were more contaminated than the avocado form the new market.
TABLE OF CONTENTS
Title page Certification Dedication Acknowledgement List of Tables List of figures Abstract 
CHAPTER ONE Introduction 1.1Background of study 1.2Statement of problem 1.3Aim and Objectives of the study 1.4Hypothesis  1.5Significance of the study 1.6Limitation of the study
CHAPTER TWO Literature review 2.1 The origin of Avocado fruits 2.2 Uses of avocado fruits 2.3 Preservation of avocado fruits 2.4 Ways avocado fruits can be prone to contamination 2.5 Microbial spoilage of avocado fruit 2.6 Control of bacterial and other disease of avocado fruits and fruits products 2.7 Some biochemical test for bacterial isolates
CHAPTER THREE Materials and methods 3.1Sample collection 3.2Sterilization of media an material used 3.3Preparation of media 3.4Bacteria and fungi isolate from avocado fruits 3.5Bacterial isolation and identification 3.6Fungal isolation and identification 3.7Lactophenole blue staining technique for fungi isolates 3.8Staining techniques for bacteria isolates grains stain 3.9Biochemical test for identification of bacteria isolates
CHAPTER FOUR Results and discussion  4.1Bacterial Examination 4.2Fungi, examination
CHAPTER FIVE Conclusion and recommendation 5.1Conclusions 5.2Recommendation Reference