In-vivo Anti-inflammatory Activity Studies of Some P-nitrophenyl Hydrazones

The goal of this research was the discovery of newer anti-inflammatory drug. Four hydrazones with p-nitrophenyl moiety were synthesized by solvent-free and conventional synthetic methods with good to excellent yields. The synthesized hydrazones were evaluated for their anti-inflammatory activities using carrageenan-induced paw edema in mice. All the compounds indicated significant anti-inflammatory activity, demonstrating slow onset of action and longer duration of action compared to celecoxib and piroxicam; this characterized them as suitable for the treatment of chronic inflammatory diseases. Most of the compounds indicated superior anti-inflammatory activities compared to celecoxib and piroxicam after 4½ hours of inflammation induction. All the compounds demonstrated time-dependent anti-inflammatory activities. Compound 3b [1-(4-nitrophenyl)-2-(3,4,5-trimethoxybenzylidene) hydrazine] had the best activity, indicating time-dependent and dose-dependent activities during the studies. This suggests that the trimethoxy benzaldehyde moiety confers favorable pharmacokinetic properties to the compound. The hydrazones, especially 3b have been identified as lead compounds and are recommended for further in-vivo anti-inflammatory evaluations against other acute and chronic inflammatory animal models.