Hepatoprotective Effect of Methanol Extract of Diospyroschloroxylon Leaf in N-Nitrosodimethylamine-induced hepatotoxicity in rats

ABSTRACT  

The  present  study  investigated  the  ameliorative  properties  of  Diospyroschloroxylon  (methanolic)  leaf  extract (DCLE)  in  liver  of  rats  intoxicated  with  N-Nitrosodimethylamine  (NDMA).  Antioxidant  markers-  Superoxide dismutase  (SOD),  catalase  (CAT),  reduced  glutathione  (GSH)  and  lipid  peroxidation  were  assayed spectrophotometrically. 

DNA  fragmentation  was determined spectrophotometrically. The Bcl-2, Ki-67 and  CD15 markers  were  assayed  using  immunohistochemistry.  The  activities  of  SOD  and  catalase  were  decreased  in  the NDMA-treated rats by 99.5% and 47.1%, respectively compared with controls, whereas pretreatment with DCLE showed significantly elevated (p < 0.05) values as against NDMA. NDMA treatment reduced GSH level by 35.7% compared with controls, while DCLE pretreatment significantly increased the level relative to NDMA group. The NDMA  significantly  increased  (p