BCH 303 - Introduction to Enzymology

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Enzyme Assay, Purification and Cell Disruption

Hexokinase and glucose oxidase

Uricase and cholesterol esterase

NEED FOR ENZYME PURIFICATION

STEPS IN ENZYME PURIFICATION

Microbial source

Heterologous enzymes in microbes

Plant source

Animal source

Methods for initial recovery

Removal of cell debris and whole cells

Removal of nucleic acids and lipids

Concentration of enzymes

Final purification by column chromatography