BCH 303 Enzyme kinetics

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Introduction

Basis

Michaelis-Menten equation

Assumption of equilibrium

Assumption of steady state

Significance of Km

Analysis of Kinetic Data

Lineweaver-Burk Plot

Lineweaver-Burk equation

Interpreting the LB plot

Eadie-Hofstee plot

Eadie-Hofstee equation

Hanes plot

Hanes–Woolf equation