ALTERNATIVE STAINING OF KIDNEY TISSUE SECTIONS WITH EXTRACTS OF TUMERIC (Curcuma Longa) AND ZOBO (Hibiscus Sabdariffa)

Abstract

Most stains used in histopathology are synthetic, toxic and costly for use. Hence, this study was carried out at the Histopathology Laboratory of Imo State University to find out the probability of replacing routine toxic and costly stains for nature, non-toxic, less costly and effective stains. Sixteen (16) kidney tissue sections were used for this study obtained from the Federal Medical Center, Owerri of which was divided into two groups of eight (8) kidney sections respectively. This division was according to stains to be used on both sections either with Haematoxylin and Eosin or prepared adjusted PH extracts of Zobo (H. sabdariffa) and Turmeric (C.Longa) viz: The group 1 (8 kidney sections) were stained with Haematoxylin and Eosin, group 2 (8 kidney sections) stained with Zobo (H. sabdariffa) and  Turmeric  (C.Longa)  After  analysis  it  was  concluded  that  Zobo  (H.  sabdariffa)  and Turmeric (C.Longa) were not good alternative stains. Keywords: Staining, Extracts, Tumeric, Zobo.